Wishes

I looked in the sky and there I saw a star shining so bright above
I closed my eyes and wished upon the star that I would fine true love
Someone who needed me
Someone to share my life
For a love that would be true
I would wait forever
So...no...matter how long it may be
I will be waiting
One star-brighter than the others
Two Hearts-beating for each other
I believe wishes really come true
Love at first sight I knew it from the moment when you said hello
I hoped you felt it too, but we were both so shy-how was I to know
when you reached for my hand
I knew you were the one
We laughed and talked for hours like I'd known you forever

Like...a...dream or something from a book
True love had found me
One star-brighter than the others
Two Hearts-beating for each other
Now I see wishes really come true
You just have to dream
Nothing's as bad as it seems
to be...believe me
Someone's waiting for you to try
There in the sky
One star-brighter than the others
Two Hearts-beating for each other
You will see wishes really come true
You can't stop believing-wishes do come true
You gotta believe me (wish on a star) wishes do come true...

Chromatin immunoprecipitation

Chromatin immunoprecipitation (ChIP), to study DNA-protein interaction.

http://www.bioscience.org/2008/v13/af/2733/fulltext.asp?bframe=figures.htm&doi=yes

Phage display

Phage display, displaying peptides on the surface of bacteriophage.
DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein
http://www.bio.anl.gov/molecular_and_systems_biology/antibody.html


The growth and potential of human antiviral monoclonal antibody therapeutics

Tandem-affinity purification

Tandem-affinity purification, used to study protein-protein interaction.

http://www.nature.com/nrm/journal/v4/n1/fig_tab/nrm1007_F1.html

Measurement of protein C

Protein C, also known as autoprothrombin IIA and blood coagulation factor XIV,[1]:6822[3] is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death, and maintaining the permeability of blood vessel walls in humans and other animals. Activated protein C (APC) performs these operations primarily by proteolytically inactivating proteins Factor Va and Factor VIIIa. APC is classified as a serine protease as it contains a residue of serine in its active site.[4]:35 In humans, protein C is encoded by the PROC gene, which is found on chromosome 2.

Principles
Protein C may be measured by:
a) Immunological by means of an ELISA assay Remember this measures only the amount of Protein C present and NOT its functional activity.

b) A clot-based functional APTT assay - the time to clot formation after addition of a Protein C activator is determined and from this the amount of Protein C present can be determined.

c) A Chromogenic assay - Protein C is activated using (commonly) Protac™, an extract of the venom of Akistrodon contortrix contortrix and the concentration of Protein C is determined from the rate of colour change in the test sample due to cleavage of a chromogenic substrate.

d) A thrombin-generation-based test has also been shown to detect Protein C deficiency.

Method

Commercial kits are readily available for Protein Assays and should use a reference standard calibrated against the current International Standard for Protein C.

1. ELISA Assays: Most ELISA assays use either monoclonal or polyclonal antibodies against Protein C. 

2. Functional, Clotting-based Protein C Assays: These are based on either the PT or the APTT, although the APTT is more commonly used. Patient platelet poor plasma is incubated at 37°C with phospholipid, a contact activator (e.g. Kaolin) and a Protein C activator (e.g. Protac). After incubation (typically 1-4 minutes) calcium is added to initiate clotting. The time taken to form a clot is timed. From this the Protein C level is determined from a reference curve. 

The clotting time of the APTT [or PT] will be influenced by the amount of Va or VIIIa present in the reaction mixture and in turn this will be influenced by the activity of the Activated Protein C [APC]. APC is generated from the conversion of Protein C to activated Protein C by Protac. So if there is a reduction in circulating Protein C levels, then less APC will be generated, less Va and VIIIa will be inactivated and the clotting times will be shorter.

http://www.practical-haemostasis.com/Thrombophilia%20Tests/pc_assays.html

愛・おぼえていますか

Right now, I hear your voice
saying "Come here to me."
Just when it seemed loneliness had beaten me.
Right now, I see you
walking over to me.
I close my eyes and wait for you to come.

Till yesterday, it seemed filled only with tears.
But now my heart is

* Do you remember? The time when our eyes first met?
Do you remember? The time when our hands first touched?
That was the very first time I set out on the journey of love.
I love you so.

Right now, I can feel your gaze
though you're not here with me.
And in my body a warmth begins to glow.
Right now, I believe in your love,
so won't you please
watch over me from so far away?

Till yesterday it seemed filled only with tears.
Now the world is...

從沒這麼愛戀過

從來沒有這麼愛戀過　每次當妳親我
猶如著魔　仍然是妳笑聲最可愛
我願天天跟妳　纏綿地過

像妳會散發某些香氣　令我一次又一次動心
或我說不清　或我說不對　仍然是最真

我也許不懂妳心　我也許貌不如人
也許妳不會明白我心　我也許不懂說謊
但我可撫心自問　我清楚知道
如何待妳好 I LOVE YOU AH...

從無人令我如此興奮　再沒不快事情
如沐夜風　從無人令我如此牽掛
每日心似亂　如迷路中

我也許不必再等　我身邊是心中人
我竟找到妳　同渡下半生 I LOVE YOU