Sunday 31 March 2013

immobilized metal-affinity chromatography (IMAC)

IMAC is based on the interactions between a transition metal ion (Co2+, Ni2+, Cu2+, Zn2+) immobilized on a matrix and specific amino acid side chains. Histidine is the amino acid that exhibits the strongest interaction with immobilized metal ion matrices, as electron donor groups on the histidine imidazole ring readily form coordination bonds with the immobilized transition metal.

e.g. Polyhistidine-tag column

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Monday 25 March 2013

Jenner's Stain

Jenner's Stain (methylene blue eosinate) is used in microscopy for staining blood smears.

Wright's stain

Wright's stain is a histologic stain that facilitates the differentiation of blood cell types. It is used primarily to stain peripheral blood smears and bone marrow aspirates which are examined under a light microscope. In cytogenetics it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.

Warthin–Starry stain


The Warthin–Starry stain (WS) is a silver nitrate-based staining method (a silver stain) used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin (1866-1931) and Allen Chronister Starry (1890-1973), for the detection of spirochetes.[1][2] It has been considered the best stain for detection of spirochetes,[3] and is also used to stain Helicobacter pylori, Lawsonia intracellularis,[4] Microsporidia,[5][6] and particulates.[7]
WS stains organisms dark brown to black, and the background light golden brown/golden yellow.

Carbol fuchsin


Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. It is commonly used in the staining of mycobacteria as it has an affinity for the mycolic acids found in their cell walls.
It is a component of Ziehl-Neelsen stain.  Carbol fuchsin is used as a dye to detect [acid fast bacteria] because it is more soluble in the cells wall lipids than in the acid alcohol. If the bacteria is acid fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol.
Carbol-fuchsin is also used as a topical antiseptic.

Dieterle stain


The Dieterle stain is a way of marking tissue for microscopic examination.
It is used to find the organisms that cause cat-scratch disease and syphilis and sensitive for mycobacterium tuberculosis.

Treponema pallidum particle agglutination assay


The treponema pallidum particle agglutination assay (also called TPPA test) is an indirect agglutination assay used for detection and titration of antibodies against the causative agent of syphilis, Treponema pallidum subspecies pallidum.
In the test, gelatin particles are sensitized with T. pallidum antigen. Patient serum is mixed with the reagent containing the sensitized gelatin particles. The particles aggregate to form clumps when the patient serum is positive for syphilis. In other words, the patient's serum contains antibodies to T. pallidum. A negative test shows no clumping of gelatin particles. This is a type of specific treponemal test for syphilis.

Rapid plasma reagin


Rapid Plasma Reagin (RPR) refers to a type of test that looks for non-specific antibodies in the blood of the patient that may indicate that the organism (Treponema pallidum) that causes syphilis is present. The term "reagin" means that this test does not look for antibodies against the actual bacterium, but rather for antibodies against substances released by cells when they are damaged by T. pallidum.
In addition to screening for syphilis, an RPR level (also called a "titer") can be used to track the progress of the disease over time and its response to therapy.

Friday 8 March 2013

Sailor Stars Song


Give sadness now a sailor smile
Bringing a miracle, a sailor wing
Everybody carries a shining star inside
I won't give up! To tomorrow, a sailor yell
For sure! I will catch it! The sailor star
Let this vow sound through the whole galaxy

After the time when you disappeared from me
I began my journey searching for you
The start is on the map, the stenciled picture of an angel
The destination it points to, a waiting dark colisseum
In my trembling heart, the secret kiss of that day
However hard destiny may be
I will keep following it

I won't look back, with my sailor eyes
Reaching out to you, with my sailor wind
This song is the guidepost of the stars
I won't give up! To tomorrow, a sailor yell
For sure! I will find it! The sailor star
With the wings of an angel, I take off

Alone I run along the road of the unknown
At last I have arrived, here at this fort
At the bottom of the flask, which you left behind
A single piece of the star of trial, now chant the magic spell

This is our miraculous destiny
The past and the future, crossing them all, to catch up with you

Give sadness now my sailor eyes
Bringing a miracle, a sailor wing
Everybody carries a star of fate inside
I won't give up! To tomorrow, a sailor yell
For sure! I will catch it! The sailor star
Let this vow sound through the whole galaxy

I won't look back, with my sailor eyes
Reaching out to you, with my sailor wind
This song is the guidepost of the stars
I won't give up! To tomorrow, a sailor yell
For sure! I will find it! The sailor star
With the wings of an angel, I take off