我與你

昨日：
當我這天回憶　共你年月裡認識　無盡甜夢你為我織
心靈留下痕跡　是你常令我獲益　漫漫長路有你　讓我懂得愛惜
共經過　數不清的波折　然後又經過　祝福與道別
然後又經過　想不到的轉折　然後再一次相遇時　情是更加熱
天　仍然是藍色　熱愛　無用再著跡　濃情　原是永沒褪色
想到明日回憶　就算　離別你在即　仍然是愛你　一生都衹會這樣說

PCR techniques

Some of the 32 (known?) PCR techniques:
·       Allele specific PCR,
·       Alu PCR,
·       Asymetric PCR,
·       Colony PCR,
·       Competitive quantitative PCR.
·       Competitive RT-PCR (c-RT-PCR).
·       Differential display RT-PCR.
·       Hot start PCR,
·       Immuno-PCR on chip
·       In Situ PCR.
·       Inverse PCR,
·       ISSR PCR,
·       Late PCR,
·       Ligation anchored PCR (LA-PCR).
·       Long PCR,
·       Long PCR,
·       Marathon RNA PCR.
·       Multiplex PCR,
·       Multiplex PCR.
·       Nested PCR,
·       Non-competitive RT-PCR.
·       PCR cloning,
·       PC sequencing
·       RACE-PCR.
·       RAP-PCR (RNA arbitrarily primed PCR).
·       Real time PCR
·       RNase ligase mediated PCR (RLM_RACE PCR).
·       RT PCR,
·       Standard PCR,
·       Suppression subtraction Hybridization PCR (SSH).
·       Touch down PCR,
·       AFLP PCR,
·       Single cell PCR

454 pyrosequencing

As the process continues, the complementary DNA strand is built up and the nucleotide sequence is determined from the signal peak in the pyrogram (intensity).

actual sequence: GCAGGCCT


http://genoseq.ucla.edu/action/view/Pyrosequencing

Amplification Refractory Mutation System (ARMS)

also called allele-specific PCR (ASP)
to amplify or detect mutant DNA, use 3 primers

ARMS has the ability to isolate low levels of a mutant sequence in a background of wild-type DNA. The system depends on the specificity of a primer for the normal sequence and another primer for the mutation.

In ARMS, the primer pair is designed so that one of the 3' ends coincides with a variant nucleotide in the target sequence. When the primer mismatches the template the frequency of extension is very low and consequently the effective number of sequence copies available for amplification is greatly reduced.

The principle of ARMS. Primers are designed to amplify wild-type and mutant sequence. The difference between these two primers is the 3' base, where one matches wild-type sequence and the other the mutant. The reaction containing the mutant primer will only extend mutant template and wild-type template will not be extended by this primer. (3' base of primer is where extension begins, therefore need specificity)

http://www.horizonpress.com/pcr/pdf/rtpcr/rtpcr09.pdf
http://clincancerres.aacrjournals.org/content/12/13/3875/F1.large.jpg

high resolution melting analysis

high resolution melting analysis (HRM):
-used to detect SNP
-must use a saturating dye (e.g. Evagreen)
-after real-time PCR, normalize a melt curve, suppress the curve that differs the most.

Methylation Sensitive (MS)-HRM:

-Bisulfite treatment of DNA converts unmethylated Cytosine to Uracil.
— Methylated Cytosine are protected and remain intact.

-After PCR amplification all Uracils are converted Thymine.

-perform DNA sequence analysis, if T, that means the DNA is unmethylated.
if C, the DNA is methylated.

RNA extraction using trizol

Principle reagents:
guanidinium isothiocyanate (powerful protein denaturant) -> inactivation of RNases
acidic phenol/chloroform(toxic) -> partitioning of RNA into aqueous supernatant for separation

Note: low pH is crucial since at neutral pH DNA not RNA partitions into the aqueous phase.


Nucleic acid (RNA, DNA) partitions in the aqueous phase, while protein partitions in organic phase.

For RNA purification, the pH is kept at around pH 4, which retains RNA in the aqueous phase preferentially. For DNA purification, the pH is usually near 7, at which point all nucleic acids are found in the aqueous phase.


Acid phenol specifically leaves RNA in the aqueous phase.  As the pH decreases, the concentration of protons increases.  DNA carries a negative charge because of the phosphate groups in its sugar-phosphate backbone, which are neutralized in acid by protonation.  In this case, DNA dissolves in the organic phase (like dissolves like).  RNA, on the other hand, is not neutralized in acid because, even though it also has a negative charge, it has exposed nitrogenous bases (it is single-stranded), which can form hydrogen bonds with water, keeping it in the aqueous phase.

http://physiology.med.cornell.edu/faculty/mason/lab/zumbo/files/PHENOL-CHLOROFORM.pdf

thymol gargle

thymol gargle, for mouth wash, anticeptic

hemocytometer

http://celeromics.com/en/resources/docs/Articles/Cell-counting-Neubauer-chamber.pdf

Neubauer Chamber Cell Counting using a hemocytometer

hepatic encephalopathy

7 Dec, 2012
12A ward round
? hepatic encephalopathy
liver dysfunction, toxin affect brain
one of the symptoms: jerking of limbs
order blood test for ammonia level

Reasons to do OGD:
Upper GIB, epigastric pain, anemia

Wishes

I looked in the sky and there I saw a star shining so bright above
I closed my eyes and wished upon the star that I would fine true love
Someone who needed me
Someone to share my life
For a love that would be true
I would wait forever
So...no...matter how long it may be
I will be waiting
One star-brighter than the others
Two Hearts-beating for each other
I believe wishes really come true
Love at first sight I knew it from the moment when you said hello
I hoped you felt it too, but we were both so shy-how was I to know
when you reached for my hand
I knew you were the one
We laughed and talked for hours like I'd known you forever

Like...a...dream or something from a book
True love had found me
One star-brighter than the others
Two Hearts-beating for each other
Now I see wishes really come true
You just have to dream
Nothing's as bad as it seems
to be...believe me
Someone's waiting for you to try
There in the sky
One star-brighter than the others
Two Hearts-beating for each other
You will see wishes really come true
You can't stop believing-wishes do come true
You gotta believe me (wish on a star) wishes do come true...

Chromatin immunoprecipitation

Chromatin immunoprecipitation (ChIP), to study DNA-protein interaction.

http://www.bioscience.org/2008/v13/af/2733/fulltext.asp?bframe=figures.htm&doi=yes

Phage display

Phage display, displaying peptides on the surface of bacteriophage.
DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein
http://www.bio.anl.gov/molecular_and_systems_biology/antibody.html


The growth and potential of human antiviral monoclonal antibody therapeutics

Tandem-affinity purification

Tandem-affinity purification, used to study protein-protein interaction.

http://www.nature.com/nrm/journal/v4/n1/fig_tab/nrm1007_F1.html

Measurement of protein C

Protein C, also known as autoprothrombin IIA and blood coagulation factor XIV,[1]:6822[3] is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death, and maintaining the permeability of blood vessel walls in humans and other animals. Activated protein C (APC) performs these operations primarily by proteolytically inactivating proteins Factor Va and Factor VIIIa. APC is classified as a serine protease as it contains a residue of serine in its active site.[4]:35 In humans, protein C is encoded by the PROC gene, which is found on chromosome 2.

Principles
Protein C may be measured by:
a) Immunological by means of an ELISA assay Remember this measures only the amount of Protein C present and NOT its functional activity.

b) A clot-based functional APTT assay - the time to clot formation after addition of a Protein C activator is determined and from this the amount of Protein C present can be determined.

c) A Chromogenic assay - Protein C is activated using (commonly) Protac™, an extract of the venom of Akistrodon contortrix contortrix and the concentration of Protein C is determined from the rate of colour change in the test sample due to cleavage of a chromogenic substrate.

d) A thrombin-generation-based test has also been shown to detect Protein C deficiency.

Method

Commercial kits are readily available for Protein Assays and should use a reference standard calibrated against the current International Standard for Protein C.

1. ELISA Assays: Most ELISA assays use either monoclonal or polyclonal antibodies against Protein C. 

2. Functional, Clotting-based Protein C Assays: These are based on either the PT or the APTT, although the APTT is more commonly used. Patient platelet poor plasma is incubated at 37°C with phospholipid, a contact activator (e.g. Kaolin) and a Protein C activator (e.g. Protac). After incubation (typically 1-4 minutes) calcium is added to initiate clotting. The time taken to form a clot is timed. From this the Protein C level is determined from a reference curve. 

The clotting time of the APTT [or PT] will be influenced by the amount of Va or VIIIa present in the reaction mixture and in turn this will be influenced by the activity of the Activated Protein C [APC]. APC is generated from the conversion of Protein C to activated Protein C by Protac. So if there is a reduction in circulating Protein C levels, then less APC will be generated, less Va and VIIIa will be inactivated and the clotting times will be shorter.

http://www.practical-haemostasis.com/Thrombophilia%20Tests/pc_assays.html

愛・おぼえていますか

Right now, I hear your voice
saying "Come here to me."
Just when it seemed loneliness had beaten me.
Right now, I see you
walking over to me.
I close my eyes and wait for you to come.

Till yesterday, it seemed filled only with tears.
But now my heart is

* Do you remember? The time when our eyes first met?
Do you remember? The time when our hands first touched?
That was the very first time I set out on the journey of love.
I love you so.

Right now, I can feel your gaze
though you're not here with me.
And in my body a warmth begins to glow.
Right now, I believe in your love,
so won't you please
watch over me from so far away?

Till yesterday it seemed filled only with tears.
Now the world is...

從沒這麼愛戀過

從來沒有這麼愛戀過　每次當妳親我
猶如著魔　仍然是妳笑聲最可愛
我願天天跟妳　纏綿地過

像妳會散發某些香氣　令我一次又一次動心
或我說不清　或我說不對　仍然是最真

我也許不懂妳心　我也許貌不如人
也許妳不會明白我心　我也許不懂說謊
但我可撫心自問　我清楚知道
如何待妳好 I LOVE YOU AH...

從無人令我如此興奮　再沒不快事情
如沐夜風　從無人令我如此牽掛
每日心似亂　如迷路中

我也許不必再等　我身邊是心中人
我竟找到妳　同渡下半生 I LOVE YOU

愛到分離仍是愛

女: 風 吹過萬里沙 途中是我碰著途中的你
也許只因眼中 都有孤單的倒影

男: 走 方向在那邊 尋找是我也在尋找的你
夜了半夢還是半醒 某種感觸便再出說不清

女: 難說出應不應該 跟你在這刻濃濃的愛
試問人在旅途時 愛下去可有未來

男: 難說出應不應該 跟你在這刻全無驅策的愛
你若停在我的路 你會否仍然是你

女: 飄 飄過上半生 誰知又會向著何方飄去
到底哭聲笑聲 本來都祗一瞬間

男: 不 不要問結果 明天共你回望這天一切

合: 又會笑著還是痛哭 到底這宵 便更要去珍惜

女: 誰再管應不應該 只要在這刻濃濃的愛
即是人在旅途時 永沒法肯定未來

男: 誰再管分不分開 只要在這刻全無拘束的愛
世上原是太多路 愛到分離仍是愛

合: 誰再管應不應該 只要在這刻濃濃的愛
即是人在旅途時 永沒法肯定未來
誰再管分不分開 只要在這刻全無拘束的愛
世上原是太多路 愛到分離仍是愛

誰再管應不應該 只要在這刻濃濃的愛
世上原是太多路 愛到分離仍然是愛

Reality

Met you by surprise, I didn't realize that my life would change for ever
Saw you standing there, I didn't know I'd care
There was something special in the air

Dreams are my reality, the only kind of real fantasy
Illusions are a common thing, I try to live in dreams
It seems as if it's meant to be
Dreams are my reality, a different kind of reality
I dream of loving in the night and loving seems all right
Although it's only fantasy

If you do exist, honey, don't resist, show me your new way of loving
Tell me that it's true, show me what to do
I feel something special about you

Dreams are my reality, the only kind of reality
Maybe my foolishness is past, and maybe now at last
I'll see how the real thing can be
Dreams are my reality, a wondrous world where I like to be
I dream of holding you all night and holding you seems right
Perhaps that's my reality

Met you by surprise, I didn't realize that my life would change for ever
Tell me that it's true, feelings that are new
I feel something special about you

Dreams are my reality, a wondrous world where I like to be
Illusions are a common thing, I try to live in dreams
Although it's only fantasy
Dreams are my reality, I like to dream of you close to me
I dream of loving in the night and loving you seems right
Perhaps that's my reality.

幸せのドア（幸福之门）
中西保志&沢田知可子

君の手を握りしめて 歩き出した 银色の道
握着你的手,走出银色的路

あどけない 君の笑颜
你的笑脸天真烂漫

夜明け前を照らしながら
在黎明之前

贵方とならば どんな道のりも楽しむから
只要能和你在一起,怎样的路都是享受
幸せになろうね
真幸福

誓う言叶 かみしめながら
回味着誓言

静かな町を目覚めてく 空を何処まても行ける気がする
安静的街道苏醒了,仿佛能走到天上去

幸せのドアだね
那是幸福之门啊

幼い日 梦见ていた
小时候梦见过

ただ一つの大事な出会い
只有一次重要的相逢

贵方へと届くように 愿っていた いつも いつも
想到你那里去,经常经常

君を守れば どんな运命も爱せるから
只要能守住你,怎样的命运都喜欢

幸せになるって 二人の手が描く未来 今を大事に 大事に生きよう
用两个幸福的人的手描绘未来,为此重要的活着

何でもなれる気がした 始まりのドアだね
不管会变成怎么样,那是开始之门啊

何时间歩いても 话しても 温もりを感じてる
不管走多久说多久,都感到很温暖
白い空に日が升る 绮丽だね
太阳升起,很美丽

幸せになろうね
是幸福啊

誓う言叶 かみしめながら 今を大事に 大事に歩こう
回味着誓言,重要的走到现在

この広い空に描いた 幸せのドアだね
描绘这片广阔的天空,这是幸福之门啊

Ichiman Metre No Keshiki (10,000 Metres of Scenery)

Flying towards the horizon in the afternoon, view blocked by the drifting clouds
Even though I know this love is full of mistakes, I probably won’t be able to go back
I’ve been to the stars, how far will I go? My wings are enticing me

The sky is closer than usual, I’ll rest my tired heart
I’ve had days where I’ve hugged my knees and cried, and there’ll be more
Does he like me? Does he hate me? I love him

Your attitude is whimsical, if I chase you you’ll run away, calculation is a huge crime to you
I want to yell at the top of my lungs that you’re not alone, in this deserted place
The ground is just like it is in the map, looking down on the little towns, they look perfect

My crossing time and letting you in, showing you such a weak spot
Is because in the heat of the moment I can be bold, the freedom is great, I hate being passive

The sky is closer than usual, I’ll rest my tired heart
I’ve had days where I’ve hugged my knees and cried, and there’ll be more
Our bond is getting deeper

愛しい人よ～名もなき旅人よ～

心愛的人呀～無名的旅人呀～

心愛的人呀　無名的旅人呀
好想讓破碎的心靜一靜啊

曾被某些事物所迷住　畫下那樣的夢想
曾追求過的　竟是在遙遠的過去
即使能順利地生存下去　還是會失去某些東西
雖然是在已經下定決心走這條不歸路的情況下而出走的

現今的心境　比當時更加年輕了　你當時還乘著計程車飛奔
來到我家　睡在我身旁啊

赤子之心的人呀　無名的旅人呀
好想替你連繫這破碎的心啊
不回頭　不讓步
明天　一定會放晴的吧

夕陽細長的影子　延伸到十字路口
心在長者ヶ崎奔跑啊

在你的世界裡　是否有我呢？
因為我挺直腰桿　一直跟著你一路走來…

如今　你已不再像你了　一副都會模樣
縱使那個夏天已遠去　那約定我不會忘記啊

無名的旅人呀　心愛的人呀
好想讓破碎的心靜一靜啊
不回頭　不讓步
明天　一定會放晴的吧

無名的旅人呀　心愛的人呀
好想讓破碎的心悄悄地靜一靜啊
無法回頭　不能讓步
明天一定是晴天的吧

分手總要在雨天

晨曦細雨　重臨在這大地　人孤孤單單躲避
轉身剎那　在這熟悉的路旁　察覺身後路人是你
如一套戲　重逢在這舊地　而彼此不知怎預備
一些嘆氣　跟一串慰問　和隨便說一些讚美

為何你眼光　年月未變　思憶怎麼要再返舊年
你說要走的一晚　連綿夜雨　也似這天

總要在雨天　逃避某段從前　但雨點偏偏促使這樣遇見
總要在雨天　人便掛念從前　在痛哭擁抱告別後從沒再見
(是你的一切告別在雨天)

而一個我　言詞漸覺乏味　人不知怎麼躲避
終於看見　在這熟悉的路旁　那個他靜靜凝望你
而一個你　重離別這舊地　臨走的一刻親近地
輕輕送我　多真摯慰問　猶如逝去當天語氣

目覚めた朝は

Mezameta Asa wa...

Even in this city which I had loved so much
There's nothing I can immerse myself into
The open lot I had looked at from my veranda
Will soon be lined with buildings, changed completely

You're free to choose where you go
But at least be smiling when you appear in my dreams
Ah, on the morning I woke up, there was a gray, cloudy sky
It seemed kind of like my own feelings which would not clear

Even the things I could say because I didn't know better
When I learn too much, I find I can't say them anymore
There isn't anyone who ever showed my their hearts that much
I want you to see that my rough talking is just a front

I wonder how we could return to how we were long ago
The seasons will completely carry us away
Ah, on the morning I woke up, there was a blue rain
The first thing I thought of the entire day was your face

Ah, I have to hurry and give chase
Because you're passing right before my eyes
Ah, the train that's started running won't turn around
I had wanted to confess, that "I still love you"



醒来的早晨

曾经那么喜欢的都会
已经不再是我所向往的了
从阳台看到的空地
也即将变成林立的大楼了

虽然说要到哪去是你的自由
至少当我从梦中醒来的时候 对我笑一笑吧
啊啊 醒来的早晨是灰色的乌云密布的天空
就好像我那无法压抑的心情

因为不了解才说得出的事
也因为太过了解 反而说不出口了
没有能让我这样推心置腹的女性
好希望你能注意到 那粗暴的话只是我在逞强

要怎么做才能回到以前的两人啊？
季节已经改变了
啊啊 醒来的早晨是令人忧郁的雨
(每)一天最先想起的就是你的容颜

啊啊 不赶快追上去的话…
因为你已从我眼前行过
啊啊 已经开始奔驰的车是不会回头的
曾是那么的想说 "现在仍然爱你"

V6-change the world

I WANT TO CHANGE THE WORLD
超越疾風
一無所懼 懷抱少許的勇氣與笑容
CHANGE MY MIND
熱情源源不斷
伸手向激情的未來 一切都將光明燦爛 IT'S WONDERLAND

在灰色的天空彼方 你是否遺忘了什麼 在徬徨裡尋尋覓覓
你的心在顫抖 看不見明天的黑夜 摀起雙耳 拒絕去相信
自從遇見你 我找到了真正的歸宿
因為有你淡淡的溫柔 才使得我們覺醒

I WANT TO CHANGE THE WORLD
不再迷惘
只要能夠掌握與你的未來 我可以飛躍到任何所在
CHANGE MY MIND
熱情源源不斷
展翅向未知的明天　我們將翱翔天際 IT'S WONDERLAND
我們不斷游在同一個世界 直到實現了彼此的心願
大家都抱著相同的不安 相互扶持
停下腳步的那一刻 望向彼此 我們就在這裡

I WANT TO CHANGE THE WORLD
請別放開我的手
當我看著你呵護的眼神 我感覺自己什麼都做得到
CHANGE MY MIND
你將不再孤獨
大家都在這裡 讓我們突破萬難 IT'S WONDERLAND

I WANT TO CHANGE THE WORLD
超越疾風
一無所懼 懷抱少許的勇氣與笑容
CHANGE MY MIND
熱情源源不斷
伸手向激情的未來　一切都將光明燦爛 IT'S WONDERLAND

I want to change the world
Keep on holding on your desire
You will get that shining love
That you can't wait to know
If you go on to get your goal
It's wonderland!

I felt so worried when I spout all the time
After all, the future's not lost
Because I know that there's nothing that I really want to know.
And that I met you so I could feel
What you should know
I finally find a love
And now I'm really feeling her right
'Cause the matter is gone
This is the way to love you!

I want to change the world!
Walk again, never miss the way
Don't be afraid, I'm with you
And we could fly away
If you could stay with me forever
Change my mind!
Won't get lost if your passion's true
I'm gonna fly with you again
So you could spread your wings
And you could fly with me away
It's wonderland!

Every single thing
The time and the flyin'
Til the day, I won't let you go
You will see that your desire will be granted again
It's just the feeling
You understand the travel life
It won't be to bad to face
And then it probably you will see will not be to heart
If we could walk beside you

I Want To Change The World
Walk again, Fire against the wind
Don't be afraid to be true
Cause now you have to bring
The power of your smile in your face
Change my Mind
Keep on holding on your desire
You will get that shining love
That you can't wait to know
If you go on to get your goal
It's Wonderland!
*music*

I Want To Change The World
Walk again, never miss the way
Don't be afraid I'm with you
Now we can fly away
If you could stay with me forever
Change My Mind
Won't get lost if your passion's true
I'm gonna fly with you again
So you could spread your wings
And you will fly away with me away
It's Wonderland!

Dareka ga Matteru

My back to familiar buildings, footsteps draw near
"How have you been?" I ask innocently
It's like the time we first met
Your radiant eyes are so annoying...

With excited emotion, I decorate my heart
As I smile, someone waits for me

I was always afraid of your extravagant words
In reality, our farewell was something I smile at
The fact I loved you... I won't forget it
Even if everything became a dream

I'll give you the tenderness you treasure so much
As I look back, someone waits for me

With excited emotion, I decorate my heart
As I smile, someone waits for me

I'll give you the tenderness you treasure so much
As I look back, someone waits for me

Tooi Hoshi wo Kazoete

Though there are times when I feel
That sometimes, I don't match with you
When we both saw eye to eye over trifling things
And laughed, I forgot all that
Though I've lived in such a manner
At some point, by coincidence I realized
Though I meant to show myself
With "Yes No" I would side-step important things
And run away

More than tomorrows, count the distant stars
And hold onto those blue dreams
At those times, they'll become the star of excitement
Holding onto courage, I want to fly to you

Inside myself, there are several mes
"Because you're kind, you suffer;"
Sometimes, my optimistic self
Will scold my timid self like that

More than tears, count the distant stars
Being cool is so uncool
More than being skeptical
You're a person who lives through his emotions
When I look at you in your passion, I feel so happy

Count the distant stars
Being cool is so uncool
More than being skeptical
You're a person who lives through his emotions
When I look at you in your passion, I feel so happy

Sayonara wa Ima mo Kono Mune ni Imasu

I ride the train past a single subway station
As it cuts across this familiar town
The light in your room that time I was counting stars...
If you ever find yourself alone
And thinking of me, give me a call
Because I love you, I decided in my heart not to follow

Your farewell is still in my heart
I want to stay the way I was when we first met
The path I walked with you in my memories
I'm now following by myself

It's been a while since I've laughed this much
I'd forgotten even how to smile
Because I want to be needed by someone
I'll do my best for that someone's sake

Your farewell is still in my heart
Even though I always put on a big smile
My heart suffers with the passing of summer
The nights feel long, so long

Your farewell is still in my heart
Please stay the way you were when we first met
We had fun times, we had hard times
And someday, I'll get over you

Kono Ai ni Oyogi Tsukaretemo

Even though I'm tired of swimming in this love
I won't turn back
Until I reach you

People in love pass me by
A basketfull of dreams
While cradeling it in my arms, I walk
Kind of like the way we did back then
I turn around to face the nostalgia

Even though I'm tired of swimming in this love
Give me courage so I won't be carried away
In the rain of that weekend when we met
I felt my destiny linked with yours
It's okay, even if we get hurt... I want to love you

No matter how many romances I compare
My heart always trembles
Because you're always silent
There are even times when I feel uncertain
But I can't cry like a little girl

The way it is now
Even though I'm tired of swimming in this love
I can't turn back
A pair of footsteps
We haven't lost as much as we thought
So I'd like to become honest
More than measuring our love... I want to love you

Even though I'm tired of swimming in this love
Give me courage so I won't be carried away
In the rain of that weekend when we met
I felt my destiny linked with yours
It's okay, even if we get hurt... I want to love you

I can't tell

令人快凍僵的風兒　毫無人跡的路面崩裂
突然捎來的訊息　得知近況的我　心情被擾亂
曾經燃起的愛火　終究
會有燃燒殆盡的一天是嗎？

雖然誰都會面臨離去的時日到來　但請別忘記
因為我是如此情不自禁的喜歡你　I can't tell
我好想要碰觸　那回憶

那視線　穿透我的身體　看向遠方
開著爽朗的玩笑　但在那水面之下　你想著別的事
我邊笑邊哭著
我怎麼會這麼笨

雖然誰都會面臨離去的時日到來　但請別忘記
雖然我說不出好聽的話　I can't tell
我現在仍想要碰觸

Oh I'm gonna try and love again
Won't you stop and remember me
是的　如果我倆有著同樣的心情
因為我是如此情不自禁的喜歡你　I can't tell
我好想要碰觸
I can't tell　我好想要碰觸　那回憶

Kono Machi De Kimi To Kurashitai (I Want To Live In This Town With You)

I rub my tired eyes and get up
In the bright afternoon sunlight
Your tired face frowns
Who are you worrying about?
And since I still love you even now you’ve showed your true colours
What can I do?

You get hurt by such ordinary things
I want to put my arms around your thin shoulders

I want to live in this room with you
Let’s stay this way forever, with you being yourself
My love won’t change its shape, unwrap the inside of your heart
And fly to me

A breeze flits across my cheeks
Bringing a nostalgic fragrance
My shadow stretches out long and wraps around me gently
As I head towards a dream I won’t give up on
Every time I think of your struggles
These dazzling days become precious time

These aren’t grand words
But you’re the only dream in this whole world that I won’t give up on

I want to live in this town with you
I’ll protect you forever in my own way
There’s no logical way of explaining it, but I want to unwrap the inside of my heart
And tell you about this love

I want to live in this room with you
Let’s stay this way forever, with you being yourself

I want to live in this town with you
I’ll protect you forever in my own way
There’s no logical way of explaining it, but I want to unwrap the inside of my heart
And tell you about this love

Don't You See!

Although it'd be nice if the words would come out fluently
Like when I write a letter to a friend
What I want is more time for us to get to know each other
It's so lonely when the only ones I won't betray
Are my family; Love is asking to be loved
Though I know that I'd be better off
If I stopped believing

Don't you see!
Even though I wish and pray for miracles and memories
I'm a bit worried
Don't you see!
If I act a little disillusioned
It's because I'm afraid of being hurt

The silence while we were waiting at the taxi stop
Was only five minutes, but felt so much longer
I never thought that our relationship
Would have grown this tired and faded

Don't you see!
I'm relieved because we're the type of couple
Who won't give up because of a small fight
Don't you see!
More than seeing various other people
I always want to see the same old you

Don't you see! I'll never worry, tonight
I'll lay me down tonight.
You know, I do it for you.

Don't you see!
The smell of your town
As we walk beneath the roadside trees at dusk
Don't you see!
No matter how much of a rush
The rest of the world is in, hold on to me

Hateshinai Yume wo

From the time I escaped the revolving door
For some reason, I feel like a new me
As if murmuring that yesterday's dream will come true
The warm wind goes blowing by

Shining, the wavering stardust
Illuminates tomorrow

The endless dream
Strongly, strongly, I want to believe in it
To my heart
My overflowing courage
Always, always holding it in my heart
In my heart
Gazing at each other, let's go walking on

I always live freely as I want to
The only thing tied to me is your love
If I close my eyes, it's like yesterday again
Even on days I wandered, I was smiling

Imbued with the colors of the scenery
Let's surpass time and become shooting stars

The endless dream
Passionately, passionately I want to believe in it
To my heart
The tears welling up in my eyes
Forever, forever, holding them in my heart
In my heart
Let's go running towards happiness

The endless dream
Strongly, strongly, I want to believe in it
To my heart
My overflowing courage
Always, always holding it in my heart
In my heart
Gazing at each other, let's go walking on

The endless dream
Passionately, passionately I want to believe in it
To my heart
The tears welling up in my eyes
Forever, forever, holding them in my heart
In my heart
Let's go running towards happiness

The endless dream
Strongly, strongly, I want to believe in it
To my heart
My overflowing courage
Always, always holding it in my heart
In my heart

The endless dream
Passionately, passionately I want to believe in it
To my heart
The tears welling up in my eyes
Forever, forever, holding them in my heart
In my heart
Let's go running towards happiness

Kiraku ni Ikou

If you noticed from before
I've been glaring at a phone which won't ring
Though it's okay if you hold back a little...
We've been dating for so long
I've become unable to be honest
I heaved a sigh toward the small sky

Let's go easy now
I want to slow down those feelings I'm chasing
And lean against my my busy times...
The wind has stopped
I'm sure tomorrow, a smile will suit me

When I sat down on the concrete hugging my knees
I could see into the world
Though I'm within the sight of the crowds
Ah, I feel like I want to stay like this
After all, I've missed you

Let's go easy now
All those dreams before your eyes
Let's pick them up one by one
The one who changed
Might not have been you, but me

Let's go easy now
That tiny happiness you see reflected in the mirror
Let's pick it up one by one
The wind has stopped
Let's make memories out of our summer together

Yureru Omoi

I feel these swaying thoughts through my whole body
I want to keep walking with you, in your dreams

Summer is stealthily approaching
The glittering waves moisten the sandy beach
I'll throw away all those things I'm attatched to
Because I've decided it's you

I'd half given up on finding
A person who would match someone like me

I feel these swaying thoughts through my whole body
I want you to stay close to me like this forever
It's become clear, just like that sky
I want to keep walking with you, in your dreams

Your eyes send a signal of love
I made as if to look into them, then kissed you
Was it that I was afraid you'd see everything
That I've been running from your kindness?

That our meeting was fate is certain
And it's changed me quite a bit

I feel these swaying thoughts through my whole body
I want you to stay close to me like this forever
No matter how many lonely seasons come
I want to embrace the anxiety, in my dreams

I feel these swaying thoughts through my whole body
I want you to stay close to me like this forever
It's become clear, just like that sky
I want to keep walking with you, in our dreams

You and Me (and...)

"You promised beneath the road-side trees"
Her cheerful voice in my ears
I couldn't hide my confusion
You'll probably be spending Christmas Eve with her

I can't do it any longer, I can't pretend
These unnoticed feelings are just
Disappearing in the town as the powder snow drifts down
A harmony that's begun to disintigrate
You and me

"Think only of now," so saying, I held you
Though I felt it, my heart felt hurt
I dreamed forbidden dreams
I want to be friends with her, even now

Don't cry anymore, though love is cruel
Those warm times are so far away
I can't return to the time when we first met
A harmony that's interrupted by the wind
You and me

I can't do it any longer, I can't pretend
These unnoticed feelings are just
Disappearing in the town as the powder snow drifts down
A harmony that's begun to disintigrate
You and me

Don't cry anymore, though love is cruel
Those warm times are so far away
I can't return to the time when we first met
A harmony that's interrupted by the wind
You and me

My Friend

When I just think of you, my heart grows stronger
I'll always watch over you, so keep on running

Those single-minded dreams
Of that day so long ago are even now dazzling
No matter what worries I'm filled with
I'll believe in the straight path I'm following
I loved your plain, unadorned face...
What's sad is that it's bound to change

They were always so bright
Your eyes when you were a child, my friend
When you were by me, for some reason I could be honest
I want to become the wind that will cross this distance

When it comes to true love
We certainly crossed over various things
So that the tears wouldn't fall like a parade of stars
I took a deep breath
More than the loneliness of being alone
What's sadder is the loneliness when we're together

You were always smiling
Those times when we were together were trying, my friend
When I just think of you, my heart grows stronger
I'll always watch over you, so keep on running

They were always so bright
Your eyes when you were a child, my friend
When I just thought of you, my heart grew stronger
I'll always watch over you, so keep on running

So keep on running...

ZARD-永遠

If you ever see a red fruit
Please think about me
If your mind's made up...
I think I'll become like shards of glittering glass
And just disappear
To a paradise where no one knows me

Between the two of us now
I wonder if we could see forever
If the only thing we have is love
I won't be afraid
Of losing you anymore

Did you think I was a silly girl
Who didn't know how to communicate?
Though I suddenly see you in the streets
I don't know whether or not to call out to you
What is it that I need to protect?
Recently, I've become confused about that

"Between you and me
I wonder if we could see forever"
This uphill climb seems to continue endlessly
Since that day, I've been more lonely than I could imagine...
Just fallin' of the rain

Between you and me
I wonder if we could see forever
When I pass through this gate
I'll head towards your gentle arms
Until the day when I'll dream again