出逢った頃のように

My love is forever
Like when we first met
Even when the seasons change
It will never fade

I'm getting tired
Of love manuals and horoscopes
And I'm slowly beginning to get impatient
With everyone around me changing
I mark the days we can meet in my diary
It's kind of strange
I'm even crazier about you than before
Is it a summer love magic?

My love is forever
Like when we first met
I want to be with you forever
I treasure this love
Your clear blue eyes
Make my heart beat faster
Even when the seasons change
It will never fade

I wonder how long we talked on the phone
It still wasn't enough, was it?
What should I wear for our date tomorrow?
It's strange how worried I am

You're always close to me
Watching over me
It makes me feel as tender
As the white waves
I embrace
My growing love and joy
Yeah, I can believe
That you'll still be here next year

My love is forever
Like when we first met
I want to be with you forever
I treasure this love
Your clear blue eyes
Make my heart beat faster
Even when the seasons change
It will never fade

留住這時光

讓每點陽光　灑於你臉龐
令你的微笑　比花更盛放
誰亦會沉醉　此刻對望
常令我忘了今天在何方

愉快的時光　只因你在旁
令我想念你　與這地方
還用你誠意　將心照亮
留住我不想他往

Little pooh bear

PCR-ESI-MS

http://hawaii.gov/health/laboratories/sld-forms/np-2009_AMP_Poster_Leptospirosis_19F.pdf

use broad range PCR primer pairs e.g. for 16S rRNA conserved region
do PCR->generate a mass spectrum->base composition as a signature to determine microbes.

Anti-dsDNA Antibody by immunofluorescence

Crithidia luciliae
•a giant mitochondion containing dsDNA (kinetoplast)
•dose not contain any other antigens present in the cell nuclei

•Indirect immunofluorescence
•Crithidia lucialiae as substrate
•Positive for anti-dsDNA Ab

Biotinidase assay

• Biotinidase cleaves the amide bond of biotinyl-paminobenzoic
acid (B-pABA, an artificial substrate) to
give free biotin and p-aminobenzoic acid (pABA)

• The pABA is diazotized and reacted with a naphthol
reagent to give a purple product which can be
quantitated spectrophotometrically at 546nm

• Biotinidase activity is expressed as nmol of pABA
liberated per minute per ml serum

• Biotinidase deficient cases <0.7 nmol pABA/min/ml

immobilized metal-affinity chromatography (IMAC)

IMAC is based on the interactions between a transition metal ion (Co2+, Ni2+, Cu2+, Zn2+) immobilized on a matrix and specific amino acid side chains. Histidine is the amino acid that exhibits the strongest interaction with immobilized metal ion matrices, as electron donor groups on the histidine imidazole ring readily form coordination bonds with the immobilized transition metal.

e.g. Polyhistidine-tag column